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. 2019 Feb 12;21(6):742–754. doi: 10.1093/neuonc/noz038

Fig. 5.

Fig. 5

SND1 accelerates the GBM cell cycle, proliferation, and invasion in vitro via upregulating RhoA. (A) Western blot results of SND1, RhoA, CCND1, CCNE1, CDK4, and CDKN1B in U118MG and primary GBM cells of the control (scramble) and SND1-knockdown (SND1-sh1, sh2) groups. (B) Cell cycle analyses by flow cytometry of the cells described above. (C) Quantitative RT-PCR detection of SND1 and RhoA mRNA levels in U118MG and primary GBM cells of the scramble/vector, SND1-sh1/vector, SND1-sh2/vector, scramble/RhoA, SND1-sh1/ RhoA, and SND1-sh2/ RhoA groups. (D) Western blot results of SND1, RhoA, CCND1, CCNE1, CDK4, and CDKN1B in the cells described in (C). (E) Results of transwell invasive cell counts of the cells as indicated in (C). (F) MTT proliferation assay of the cells described above. *P < 0.05, **P < 0.01.