Table 2.
Characterization of PA loaded CS–SD NPs including mean particle size, PDI, zeta potential, EE% and drug LC%. The ratio of CS to SD for each formulation is also presented in addition to the concentration of the stabilizing agent (PVA) in preparation solutions
| Formulation No. |
CS:SD ratios |
Conc. of PVA (mg/100 mL) |
Mean particle size (nm)* |
PDI | Zeta potential (mV) | EE% | LC% |
|---|---|---|---|---|---|---|---|
| F1 | 1:1 | 0.0 | 976±43 | 1.285 | +48.4 | 58.4±2.0 | 24.6±1.9 |
| F2 | 1:2 | 0.0 | 754±34 | 1.325 | +42.7 | 51.9±2.5 | 21.4±1.5 |
| F3 | 1:3 | 0.0 | 556±52 | 1.184 | +38.6 | 45.4±3.0 | 17.9±1.4 |
| F4 | 1:5 | 0.0 | 480±28 | 1.396 | +35.2 | 36.7±2.9 | 13.9±0.9 |
| F5 | 1:1 | 0.1 | 876±56 | 0.958 | +37.6 | 56.8±1.6 | 23.9±1.5 |
| F6 | 1:2 | 0.1 | 732±48 | 0.835 | +35.5 | 51.2±1.8 | 21.6±0.7 |
| F7 | 1:3 | 0.1 | 568±47 | 0.634 | +33.1 | 46.9±2.0 | 18.4±1.8 |
| F8 | 1:5 | 0.1 | 462±19 | 0.942 | +28.7 | 37.4±1.9 | 15.2±1.0 |
| F9 | 1:1 | 0.3 | 786±42 | 0.723 | +33.7 | 57.7±1.3 | 23.7±0.7 |
| F10 | 1:2 | 0.3 | 696±32 | 0.656 | +32.5 | 51.4±1.6 | 22.9±0.6 |
| F11 | 1:3 | 0.3 | 392±18 | 0.598 | +29.5 | 46.3±1.5 | 18.5±0.75 |
| F12 | 1:5 | 0.3 | 321±22 | 0.454 | +28.8 | 36.7±0.9 | 14.7±0.9 |
Note: *STD of the mean of three determinations.
Abbreviations: CS, chitosan; SD, sodium deoxycholate; PVA, polyvinyl alcohol; PA, prednisolone; PDI, polydiversity index; EE%, encapsulation efficiency; LC%, loading capacity.