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. Author manuscript; available in PMC: 2019 Nov 6.
Published in final edited form as: Nat Cell Biol. 2019 May 6;21(6):700–709. doi: 10.1038/s41556-019-0318-1

Figure 1. Loss of Mettl3 leads to accumulation of HSCs and perturbed haematopoiesis.

Figure 1.

(a,b) White blood cell (WBC) (a) and platelet peripheral blood counts (b) from pIpC-treated control and Mx1-cre; Mettl3fl/fl mice (n=7 control (10–14d), n=7 Mx1-cre; Mettl3fl/fl (10–14d), n=4 control (2–3m), n=4 Mx1-cre; Mettl3fl/fl (2–3m), n=3 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(c) Bone marrow cellularity per hindlimb (n=28 control (10–14d), n=8 Mx1-cre; Mettl3fl/fl (10–14d), n=5 control (2–3m), n=6 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(d) Representative images of the spleens from Mx1-cre; Mettl3fl/fl and control mice 10 days and 3 months after pIpC treatment, as indicated.

(e) Spleen cellularity (n=8 control (10–14d), n=8 Mx1-cre; Mettl3fl/fl (10–14d), n=5 control (2–3m), n=6 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(f) Spleen HSC frequency (n=6 control (10–14d), n=5 Mx1-cre; Mettl3fl/fl (10–14d), n=6 control (2–3m), n=6 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(g) Frequencies of bone marrow Lin−Sca-1+c-Kit+ (LSK) progenitors (n=7 control (10–14d), n=6 Mx1-cre; Mettl3fl/fl (10–14d), n=6 control (2–3m), n=7 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(h) Frequency of bone marrow HSCs (n=7 control (10–14d), n=6 Mx1-cre; Mettl3fl/fl (10–14d), n=6 control (2–3m), n=7 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(i) Fold increase in Mx1-cre; Mettl3fl/fl bone marrow HSC or MPP frequency compared to littermate control frequencies at indicated times after pIpC treatment (n=6 (10–14d), n=7 (2–3m), n=4 (4m)).

(j) Frequencies of mature cell populations in the bone marrow (n=4 control (10–14d), n=4 Mx1-cre; Mettl3fl/fl (10–14d), n=5 control (2–3m), n=5 Mx1-cre; Mettl3fl/fl (2–3m), n=4 control (4m), n=4 Mx1-cre; Mettl3fl/fl (4m)).

(k) Frequency of megakaryocyte progenitors (Lineage−Sca1−cKit+CD150+CD41+) cells in the bone marrow >10 days after pIpC treatment (n=5 control, n=6 Mx1-cre; Mettl3fl/fl).

All samples are from biologically independent mice. Values are shown as individual points with mean ± s.d. P values were determined by unpaired two-sided Student’s t-test.