Fig.4. Antiretroviral assessments.
Antiretroviral activity was determined in MDM treated for 8 hours with free RPV, NRPV or EuCF-RPV particles (6.25, 12.5 and 25 μM RPV) and then infected with HIV-1ADA at a multiplicity of infection (MOI) of 0.1, at 1 and 5 days after drug loading. At 10 days after infection, the media was collected from wells and cells were washed in PBS before fixing with 4% PFA at room temperature. (A) Progeny HIV virion production was determined by HIV reverse transcriptase (RT) activity using beta scintillation spectroscopy. Stars represent statistical difference from positive controls. Statistical differences were determined using two-way ANOVA among groups; we used Bonferroni’s post hoc test to correct for multiple comparisons. **p< 0.01; ***p < 0.001; ****p < 0.0001. (B) HIV p24 staining (brown) counterstained with hematoxylin (blue) (scale bar = 200 μm).