Table 2. Enrichment of Ebola and Marburg viruses.
Data from an independent study ( Hölzer et al., 2016) are derived from RNAseq of Huh-7 cells infected with Ebola virus (EBOV), Marburg virus (MARV), or none (Mock). The data were re-analyzed here using SDB1. Subtraction enriched the EBOV complement to at least 57% in the EBOV-infected samples. Subtraction enriched the MARV complement to at least 39% in the MARV-infected samples. The apparent false positive enrichments can be discounted by applying a minimum requirement for 0.10% viral reads out of initial reads.
| (A) | (B) | (C) | (D) | (E) | (F) | ||
|---|---|---|---|---|---|---|---|
| Virus | Time | Initial
trimmed reads (millions) |
Portion of
initial mapped to SDB1 |
Portion of
initial mapped to EBOV |
Portion of
non-SDB mapped to EBOV |
Portion of
initial mapped to MARV |
Portion of
non-SDB mapped to MARV |
| EBOV | 03h | 32.87 | 99.70% | 0.16% | 57.17% | 0.00% | 0.12% |
| EBOV | 07h | 46.62 | 97.47% | 2.34% | 94.41% | 0.00% | 0.01% |
| EBOV | 23h | 50.41 | 62.18% | 37.29% | 99.56% | 0.00% | 0.00% |
| MARV | 03h | 41.81 | 99.76% | 0.01% | 3.62% | 0.08% | 38.96% |
| MARV | 07h | 45.81 | 99.06% | 0.03% | 3.03% | 0.63% | 69.49% |
| MARV | 23h | 34.37 | 97.61% | 0.02% | 0.87% | 1.71% | 75.45% |
| Mock | 03h | 38.55 | 99.82% | 0.02% | 10.79% | 0.00% | 0.20% |
| Mock | 07h | 36.03 | 99.86% | 0.01% | 7.59% | 0.00% | 0.18% |
| Mock | 23h | 36.99 | 99.80% | 0.02% | 9.84% | 0.00% | 0.16% |