Fig. 2.

Effects of tedizolid and comparator antibiotics on S. aureus PVL toxin expression. S. aureus strains were cultured in the presence of 1/16× MIC, 1/8× MIC, 1/4× MIC or vehicle control for 12 and 24 h. Levels of LukS-PVL were quantified from filter-sterilized culture supernatants by ELISA. Each panel shows LukS-PVL expression levels following treatment with the indicated antibiotic in (a–c) MRSA 1560 and in (d–f) S. aureus FPR3757. Bars represent average toxin expression±standard error from three biological replicates. Asterisks denote statistical significance of (*) P<0.05, (**) P<0.01, (***) P<0.001 and (****) P<0.0001 compared to the no treatment (NT) vehicle control at each corresponding timepoint.