Table 2. Primer sequences used to analyze the levels of the genes of interest and reference genes.
| Forward (5′-3′) | Reverse (3′-5′) | |
|---|---|---|
| IL-6 Ungru et al. (2012) | CCACCTCAAATGGACCACTACTC | TTTTCAGGGCAGAGATTTTGC |
| TNFα Figueiredo et al. (2009) | AAAGGACATCATGAGCACTGAAAG | GGGCCCCCTGCCTTCT |
| CD68 Ungru et al. (2012) | CTTTGGGCCAAGTTTCTCTTGT | AAGAGGCCGAGGAGGATCAG |
| HPRT1 Bogaert et al. (2006) | GGCAAAACAATGCAAACCTT | CAAGGGCATATCCTACGACAA |
| RPL32 Bogaert et al. (2006) | AGCCATCTACTCGGCGTCA | TCCAATGCCTCTGGGTTTC |
| IL-1β# | CGGCAATGAGAATGACCTGT | GCTTCTCCACAGCCACAATG |
| LPL# | ATTGTGGTGGACTGGCTGT | GCTCCAAGGCTGTATCCCAA |
| FABP1# | CAAGATCACCATCACCACAGG | GTCACAGACTTGATGCCTTTGA |
| Chemerin# | CATGGGAGGAAGCGGAAATG | CAGCTGAGCCTGTGTCTCTA |
| NF-κB# | GCTTTGTGACAAGGTGCAGA | ACGATCATCTGTGTCTGGCA |
Notes:
Five qPCR primers were newly designed and five primers were obtained from published data.
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Designed using http://primer3.ut.ee/. The specific equine cDNA sequences were provided by http://www.ensembl.org/index.html and the generated primers were validated in http://eu.idtdna.com/calc/analyzer to confirm the absence of hairpins, homodimers and heterodimers. The designed primers were created with two different modifications for each gene of interest and the more suitable primer was selected in preliminary tests. Primers were synthesized by biomers.net GmbH.