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. 2019 Jun 7;7:e7003. doi: 10.7717/peerj.7003

Table 1. Laboratory parameters for staining and scanning.

The staining regime used was continuous therefore cumulative stain duration refers to the number of days the specimen was exposed to the staining solution in total whereas stain duration details the duration of exposure to the stain in that particular test round.

Sample Stain concentration Stain duration Cumulative stain duration Scan results Recommendation
Whole, Un-skinned 7.5% 3 days 3 days No effect on musculature Skin specimen and stain further
Whole, Skinned 7.5% 13.5 days 16.5 days Stain not fully perfused Stain further with increased concentration
Whole, Skinned 20% 7.5 days 24 days Stain not fully perfused Stain further
Whole, Skinned 20% 7.5 days 31.5 days Stain not fully perfused in the thigh Stain further, with some injection of stain into thighs and body
Whole, Skinned 20% 7.5 days 39 days Sufficient perfusion Final scan to be conducted using the following settings: 17.64 µm resolution, 50 kV, 362 µA, 1 mm Al filter