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. Author manuscript; available in PMC: 2019 Jun 10.
Published in final edited form as: ACS Chem Biol. 2017 Jan 23;12(3):692–701. doi: 10.1021/acschembio.6b00921

Figure 4.

Figure 4.

Single turnover kinetics time courses. a) A schematic of the glycosylase reaction. b) A typical 8% denaturing PAGE gel showing the conversion of substrate (S) to product (P) with increasing reaction time. c) Reaction time courses for UDG/U, hOGG1/8oxoG, and hAAG/εA systems for free duplex (closed circles) or NCP (open circles) substrates. Data were fit using nonlinear least-squares regression. Error bars represent the standard deviation (n = 3). d) Maximum product yield observed in kinetics time courses for free duplex (FD; black) and NCP (gray) substrates.