Table 2.

Kinetic parameters for DNA glycosylase reactions.

Enzyme	Substrate	kobs / min (% product)a
		Free Duplex	NCP
UDG	UOUT	35 ± 2 (99%)	5.8 ± 0.1 (99%)
	UMID	21.1 ± 0.6 (95%)	16 ± 2 (11%) 0.22 ± 0.06 (8%)
	UIN	11.2 ± 0.6 (96%)	9.5 ± 1.5 (11%) 0.3 ± 0.2 (4%)
hOGG1	8oxoGOUT	5.5 ± 0.2 (93%)	2.7 ± 0.5 (5%)
	8oxoGMID	5.6 ± 0.2 (87%)	6 ± 1 (10%)
	8oxoGIN	9.0 ± 0.3 (91%)	7 ± 2 (8%)
Fpg	8oxoGOUT	10.0 ± 0.6 (84%)	4.6 ± 0.9 (9%)
	8oxoGMID	10 ± 1 (80%)	7 ± 2 (7%)
	8oxoGIN	12.2 ± 0.6 (91%)	7 ± 2 (10%)
hAAG	HxOUT	0.222 ± 0.006 (95%)	0.080 ± 0.003 (10%)
	HxMID	0.0747 ± 0.0009 (82%)	0.082 ± 0.007 (6%)
	HxIN	0.05 ± 0.01 (82%)	0.07 ± 0.03 (5%)
	εAOUT	0.0433 ± 0.0009 (91%)	0.0111 ± 0.0002 (84%)
	εAMID	0.0315 ± 0.0007 (90%)	0.006 ± 0.001 (16%)
	εAIN	0.0338 ± 0.0002 (88%)	N.R.b
EndoIII	5OHUOUT	10 ± 1 (82%)	1.6 ± 1.0 (5%)
Fpg	5OHUOUT	0.13 ± 0.02 (82%)	N.R.b
UDG	5OHUOUT	0.008 ± 0.001 (48%)	0.007 ± 0.004 (3%)

^aError represents reported standard deviation from fitting by weighted nonlinear least-squares regression.

^bN.R.: No reaction.