Figure 2.

Nitrogen Responses in Rhizobia- and Mock-Inoculated Roots Suggests that A17 Seedlings Treated with Rhizobia Are More Responsive to External Nitrogen Than Mock-Inoculated Roots.

(A) Experimental design for transcriptomics experiment.

(B) Principal component analysis (PCA) reveals a greater difference between rhizobia- and mock-inoculated plants at time 0, and greater changes over time in the gene expression in mock-inoculated (M) than rhizobia-inoculated (R) roots.

(C) Number of DEGs in rhizobia versus mock at each time point and their associated gene ontology terms.

(D) Venn diagram representing the distribution of DEGs between rhizobia-inoculated and mock-inoculated roots over time after N treatment. Genes that are differentially regulated in rhizobia-inoculated and mock-inoculated roots; if other genes in the same family are also in the DEG list, the number is given in parentheses; n = 3 except for Rhizobia-6hNitrogen where n = 2.

See also Supplemental Tables 2 and 3.