Müller cells were harvested from WT and Sig1R−/− mice following which RNA or protein was isolated. (A) qRT-PCR was performed to investigate expression Sod1, Cat, Nqo1, Hmox1, Gstt3, Gpx1, Gpx2. (B) Western blotting was performed to 57 evaluate protein levels of SOD1, CAT, NQO1, GPX1, HMOX-1. (C) Quantification of immunoblotting data (3 independent experiments). (Figures adapted from Wang et al, 2015, with permission).