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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Prog Retin Eye Res. 2018 Aug 1;67:130–149. doi: 10.1016/j.preteyeres.2018.07.003

Fig. 11. Analysis of Nrf2 and Keapl (gene and protein) in WT and Sig1R/” Müller cells.

Fig. 11.

(A) Quantitative real-time RT-PCR analysis of Cul3, Nrf2, and Keapl mRNA from WT and Sig1R- ‘ Müller cells; data are mean ± SEM (4 independent experiments). (B) Representative immunoblotting data detecting NRF2, KEAP1 and SIG1R in proteins extracted from WT and Sig1R-- mouse Müller cells; GAPDH is the loading control. (C) Quantification of immunoblotting data (mean ± SEM; 3 independent experiments)). (D) NRF2/ARE binding activity performed in WT and Sig1R-- Müller cells. The data represent values obtained at 450nm (mean ± SEM, 3 independent experiments). (**p< 0.01, ***p<0.001.) (Figures adapted from Wang et al, 2015, with permission).