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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Bone Miner Res. 2019 Jan 28;34(4):681–698. doi: 10.1002/jbmr.3635

Figure 1: Impact of 2-week ABX treatment on the intestinal microbiota immediately and 4-weeks post-ABX treatment.

Figure 1:

12-week-old male BALB/c mice were given sterile water or ABX (ampicillin and neomycin) for 2-weeks to deplete the intestinal microbiota. A) Fecal samples, from control and 2-week ABX treated mice, were plated on agar dishes to determine number of colony forming units (cfu)(n=18–20). Additionally, bacterial 16S rRNA was analyzed to assess levels of total eubacteria (n=6). B) The experimental design used to induce post-ABX dysbiosis. C) Total eubacteria levels were assed from control and 4-week post-ABX treated mice in first experimental run (n=6–7). D) Relative abundance of bacterial taxa in control and 4-week post-ABX fecal samples (n=3). Statistical analysis performed by Student’s t-test E) PCoA plot of fecal microbiome data, Bray-Curtis analysis performed to determine significance (n=8–9). Values are average ± SEM. **** p<0.0001.