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. 2019 Jun 10;14(6):e0217580. doi: 10.1371/journal.pone.0217580

Fig 6. Silencing relies on a GATA/Myb motif shared by functional Cebpa silencers.

Fig 6

A–C. Cebpa(9). D–F. Cebpa(11). G–I. Cebpa(24). A, D, G. Schematics of the construct design showing a distal CRM (blue) and the Cebpa proximal promoter (red). B, E, H. The activity of the TF repressor sites predicted by the model for each silencer. See the legend of Fig 4F for details of the calculations, axes, and legend. 9m1, 11m1, and 24m1 refer to mutant CRMs and crosses indicate mutated sites. C, F, I. Experimentally measured activity of wildtype and synthesized mutant silencers in G1ME cells. Activity levels have been normalized to the activity of Cebpa(0). Reporter assays were performed in 10 replicates. Error bars are 95% confidence intervals. Regression plots corresponding to each bar are shown in Fig L in S1 Text.