Fig 4. Nucleotide sequence of p10 promoter leader sequence.

(A) Indicates site for a series of deletions made to the promoter sequence from *transcription initiation (mRNA CAP) and location of relevant restriction digestion sites. (B) Displays nucleotide sequence of PCR fragments made using a two-step PCR to generate a series of promoter deletions as indicated in (A). The black arrows indicate binding of primer set for the first round PCR, the dotted blue line was use for the second round PCR and was used in combination with the forward primer upstream of the p10 transcription start. The p10 coding region starts at the ATG start codon as indicated by the open arrow and p10 coding region is highlighted as grey text and underlined with a black line in (A).