Table 3.
` Details of assays used to determine mRNA expression. For, forward primer; Rev, reverse primer; bp, base pairs.
| Assay ID | Accession No. | Sequence | Product length (bp) | PCR efficiency (%) | |
|---|---|---|---|---|---|
| GAPDH | NM_001289745.2 | For: | 5ʹ- GCCTCAAGATCATCAGCAATGCCT-3’ | 104 | 98.1 |
| Rev: | 5ʹ- TGTGGTCATGAGTCCTTCCACGAT-3’ | ||||
| PPARGC1A | NM_001330751.1 | For: | 5ʹ-CAGCCTCTTTGCCCAGATCTT-3’ | 101 | 104.0 |
| Rev: | 5ʹ-TCACTGCACCACTTGAGTCCAC-3’ | ||||
| IL6 | NM_000600.4 | For: | 5ʹ-GCAGAAAAAGGCAAAGAATC-3’ | 178 | 100.9 |
| Rev: | 5ʹ-CTACATTTGCCGAAGAGC-3’ | ||||
| TNF | NM_000594.3 | For: | 5ʹ-AGGCAGTCAGATCATCTTC-3’ | 142 | 99.5 |
| Rev: | 5ʹ- TTATCTCTCAGCTCCACG-3’ | ||||
| DNMT1 | NM_001130823.2 | For: | 5ʹ-TACCTGGACGACCCTGACCTC-3’ | 103 | 94.5 |
| Rev: | 5ʹ-CGTTGGCATCAAAGATGGACA-3’ | ||||
| DNMT3a | NM_175629.2 | For: | 5ʹ-TATTGATGAGCGCACAAGAGAGC-3’ | 111 | 95.9 |
| Rev: | 5ʹ-GGGTGTTCCAGGGTAACATTGAG-3’ | ||||
| DNMT3b | NM_006892.3 | For: | 5ʹ-GGCAAGTTCTCCGAGGTCTCTG-3’ | 113 | 96.2 |
| Rev: | 5ʹ-TGGTACATGGCTTTTCGATAGGA-3’ | ||||