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. 2019 Apr 5;14(5):494–503. doi: 10.1080/15592294.2019.1600388

Figure 1.

Figure 1.

MiR-155-5p promotes HSV-1 replication. (a) HeLa cells were mock infected or infected with HSV-1 for 4 hours and harvested. The relative expression level of miR-155-5p was determined with real-time PCR. (b). HeLa cells were mock infected or infected with HSV-1 and harvested at the indicated time points. The relative expression level of miR-155-5p was determined with real-time PCR. (c) HeLa cells were transfected with miR-155-5p mimics, inhibitors or negative control for 36 hours. The relative expression level of miR-155-5p was determined with real-time PCR. (d and e). After 36 hours of transfection with the miR-155-5p mimics, inhibitors or negative control, the HeLa cells were infected with HSV-1 at an MOI of 1 for 12 hours, fixed, stained with an anti-HSV-1 glycoprotein antibody (green) and subjected to a confocal microscopy analysis. DAPI (blue) was used to stain the nuclei (d). The fluorescence signal value was measured using ImageJ software and normalized to a single cell. The data are normalized to the negative control level (e). (f) After 36 hours of transfection with the miR-155-5p mimics, inhibitors or negative control, the HeLa cells were infected with HSV-1 at an MOI of 1. The culture media were collected at the indicated time points after infection and subjected to a plaque assay. *p < 0.01.