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. Author manuscript; available in PMC: 2019 Jun 10.
Published in final edited form as: Compr Physiol. 2018 Dec 13;9(1):1–58. doi: 10.1002/cphy.c170040

Table 3.

Targeted disruption of molecular targets involved in inflammation and its impact on insulin sensitivity, liver fat content, and inflammation in adipose tissue and liver in mice. Models were included if the transgenic mouse model did not have an adiposity phenotype.

Target Method Diet (%Fat) Duration Insulin Sensitivity Adipose Inflammation Liver Fat Liver Inflammation Ref
BLT-1 WBKO HFD (60%)
12 weeks
(435)
Cadherin-11 WBKO HFD (60%)
5–12 weeks
n/a (70)
Cap (Sorbs1) WBKO HFD (40% by wt)
6–8 weeks
n/a (271)
Catalase WBKO ND (18%)
21 weeks

HFD (60%)
21 weeks






(↑)
n/a


n/a
n/a


n/a
(369)
CCL2 AT Overexpression ND (6% by wt)
8 weeks

HFD (32% by wt)
8 weeks



↑ ↔





n/a


n/a
(218)
CCL2 AT Overexpression


WBKO
ND (n/a)
11 weeks

HFD (56%)
12 weeks









n/a


n/a
(221)
CCR2 WBKO HFD (60%)
24 weeks
n/a (503)
CCR2 Hematopoietic KO by BMT HFD (60%)
20 weeks
n/a (↓) (159)
CD1d (iNKT) WBKO HFD (44%)
26 weeks
(289)
CD1d (iNKT) WBKO ND (10%),
19 weeks

HFD (45%)
19 weeks






↑ ↔






(415)
CD1d (iNKT) WBKO HFD (60%)
4 days
(↓) n/a (207)
CD8 Pharmacological inhibition HFD (60%)
8 weeks
n/a n/a (345)
CD11c BMT followed by selective depletion HFD (60%)
16 weeks
↔ ↓ (374)
CD18 WBDNKI HFD (45%)
20 weeks
↔ (↑) n/a (303)
CD36 Hematopoietic KO by BMT HFD (42%)
20 weeks
n/a n/a (342)
CD95 (Fas) Adipose KO HFD (58%)
6 weeks
(↓) (520)
CXCR3 WBKO HFD (60%)
20 weeks
n/a (96)
CTRP7 WBKO HFD (60%)
12 weeks
n/a (376)
C5aR WBKO HFD (60%)
16–19 weeks
(↑) (378)
Dab2 Myeloid KO HFD (60%)
12 weeks
↔ ↑ n/a n/a (5)
FABP4 and FABP5 WBKO BMT HFD (60%)
15 weeks
↔ ↓ (↔) (136)
Fetuin-A WBKO HFD (65%)
12 weeks
n/a n/a (361)
GPR120 WBKO ND (14%)
15–20 weeks

HFD (60%)
15 weeks












(354)
HIF-1α Adipose KO HFD (60%)
10 weeks
(267)
HIF-2α WBKO (+/−) HFD (60%)
16 weeks
n/a n/a (76)
Id3 Lymphoid KO HFD (60%)
12 weeks
↔ ↓ n/a n/a (170)
Immunoglobulin μ heavy-chain WBKO
(B cell depletion)
HFD (60%)
16 weeks
↔ ↓ n/a n/a (511)
IR Myeloid KO HFD (55%)
12 weeks
n/a (297)
IFNγ WBKO HFD (60%)
13 weeks
n/a (350)
IL-10 BMT WBKO HFD (56%)
12 weeks
n/a (251)
Jα18 (iNKT) WBKO HFD (60%)
12–20 weeks
(519)
Jα18 (iNKT) WBKO HFD (32%)
18 weeks
(409)
JNK1 Hematopoietic KO by BMT HFD (60%)
20 weeks
(430)
JNK1 Hematopoietic KO by BMT HFD (60%)
26–36 weeks
↔ (↓) (481)
JNK1 Adipose KO HFD (60%)
16 weeks
↔ ↓ (403)
JNK1 Muscle KO HFD (60%)
16 weeks
(↑) (404)
JNK1 Myeloid KO HFD (60%)
4 weeks
n/a (165)
NAMPT Adipose KO ND (13%)
12–20 weeks
(446)
NLRP3 WBKO HFD (60%)
6 weeks
n/a (486)
Osteopontin WBKO HFD (60%)
25 weeks
n/a n/a (346)
Osteopontin Mostly visceral ATM in ob/ob mice ND (n/a)
5–7 weeks

(↓ osteopontin in visceral ATM)
n/a (22)
PDCD4 WBKO ND (n/a)
24 weeks
(500)
SR-A WBKO HFD (58%)
16 weeks
n/a n/a (552)
Sema3e WBKO


Overexpression
HFHS (n/a)
8 weeks

ND (n/a)
8 weeks






n/a


n/a
n/a


(425)
SOCS1 Myeloid KO ND (n/a)
12–15 weeks
n/a (405)
TNFα Mostly visceral ATM in ob/ob mice ND (n/a)
5–7 weeks

(↓TNFα in visceral ATM)
(22)
TLR2 WBKO HFD (58%)
20 weeks
(104)
TLR4 WBKO HFD (60%)
16 weeks
↔ (↓) n/a n/a (448)
TLR4 WBKO HFD (42%)
22 weeks
(↓) (380)
TLR4 Hematopoietic KO by BMT HFD (n/a)
16 weeks
(402)
TLR4 WBKO HFD (60%)
12 weeks
n/a (502)
TLR4 WBKO HFD (65%)
12 weeks
n/a n/a (361)
TLR4 Liver KO HFD (42%)
12 weeks
(208)
TLR9 WBKO HFD (60%)
12 weeks
n/a n/a (344)

Abbreviations: AT, adipose tissue; ATM, adipose tissue macrophage; BLT-1, Leukotrin B4 receptor-1; BMT, bone marrow transfer; Cap, Cbl-associated protein (Sorbs1); C5aR, complement anaphylatoxin C5a receptor; CCL2, C-C motif chemokine ligand 2 (MCP1); CCR2, C-C motif chemokine receptor type 2; CD, cluster of differentiation; CXCR3, C-X-C motif chemokine receptor 3; CTRP7, C1q/TNF-related protein 7; Dab2, disabled homolog 2; FABP, fatty acid-binding proteins; GPR120, G protein-coupled receptor 120; GH-R, growth hormone-receptor; HFD, high-fat diet; HFHS, high-fat high-sucrose diet; HIF, hypoxia-inducible factor; iNKT, invariant natural killer T cell; IR, insulin receptor; IFNγ, interferon-γ; IL, interleukin; JNK1, c-Jun NH2-terminal kinase-1; KO, knockout; NAMPT, nicotinamide phosphoribosyl-transferase; ND, normal diet (chow); NLRP3, nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing 3; PDCD4, programmed cell death-4; Sema3e, Semaphorin E; SOCS1, suppressor of cytokine signaling-1; SR-A, class A scavenger receptor; TLR, toll-like receptor; WBKO, whole body knockout; WBDNKI, whole body dominant negative knock-in