Figure 1.

TRPV1-dependent calcium responses initiated by major and minor cannabinoids. A-G.

HEK wild type and HEK-TRPV1 expressing cells were loaded with Fluo-4 and population-based calcium assays were performed in a buffer containing 1mM external CaCl₂. These are population-based (bulk Ca²⁺) measurements with each trace representing averaged triplicates of 100,000 cells per sample. After establishing a baseline for 20 sec in the presence of a matched vehicle, cells were stimulated with the indicated cannabinoid at 10 μM. H. Protocol as for A-G but with the TRPV1 ligand, Capsaicin (50 nM) as the stimulus. Capsaicin data were captured during a different experiment run and baselines vary due to dye loading differences between experiments.