Figure 8.

Cannabinoid responses in cells overexpressing nocioceptive TRPs. A-U.

HEK-TRPV2, TRPA1, or TRPM8 expressing cells were loaded with Fluo-4 and population-based calcium assays were performed in a buffer containing 1mM external CaCl₂. After establishing a baseline for 20 sec in the presence of a matched vehicle, cells were stimulated with the indicated cannabinoid at 10 μM. These are population-based (bulk Ca²⁺) measurements with each trace representing averaged triplicates of 100,000 cells per sample. V. Table 3. Summary data for Figure 8.