Skip to main content
. 2019 Apr 29;8:e45311. doi: 10.7554/eLife.45311

Table 1. Apparent diffusion coefficient D obtained from SptPALM of SsrB mutants.

No. of cells No. of tracks F1 (%) F2 (%) F3 (%) D1
(µm2s−1)
D2
(µm2s−1)
D3
(µm2s−1)
Fixed 41 496 26.9 ± 4.0 65.0 ± 3.7 8.1 ± 0.6 0.020 ± 0.002 0.066 ± 0.003 0.88 ± 0.08
WT pH5.6 89 2618 28.1 ± 2.6 34.5 ± 2.3 37.3 ± 0.5 0.041 ± 0.002 0.14 ± 0.01 1.49 ± 0.02
WT pH7.2 71 569 11.3 ± 1.1 22.2 ± 1.0 66.5 ± 1.2 0.037 ± 0.004 0.25 ± 0.03 1.53 ± 0.03
K179A pH5.6 113 1038 11.3 ± 1.3 18.7 ± 1.2 69.9 ± 1.5 0.056 ± 0.006 0.32 ± 0.05 1.70 ± 0.03
D56A pH5.6 109 1345 20.5 ± 1.4 24.0 ± 1.1 55.5 ± 1.2 0.045 ± 0.003 0.26 ± 0.03 1.61 ± 0.03
D56A pH7.2 74 384 13.0 ± 1.5 27.3 ± 1.4 59.8 ± 1.9 0.050 ± 0.005 0.31 ± 0.04 1.66 ± 0.04
D56E pH5.6 111 796 11.1 ± 0.8 16.2 ± 1.7 72.8 ± 2.2 0.053 ± 0.004 0.39 ± 0.06 1.52 ± 0.03
PAmCherry pH5.6 53 715 5.4 ± 0.3 27.8 ± 1.3 66.7 ± 1.4 0.029 ± 0.004 0.67 ± 0.04 3.61 ± 0.07
PhoP pH 5.6 81 3665 9.8 ± 1.5 23.7 ± 1.3 66.4 ± 0.4 0.033 ± 0.004 0.14 ± 0.01 1.54 ± 0.01
PhoP pH 7.2 103 2930 10.6 ± 0.6 18.8 ± 1.5 70.6 ± 1.8 0.043 ± 0.003 0.40 ± 0.04 1.58 ± 0.03
OmpR pH 5.6 43 1610 21.9 ± 1.4 20.9 ± 1.0 57.0 ± 1.3 0.054 ± 0.003 0.26 ± 0.03 1.34 ± 0.02
OmpR pH 7.2 55 2119 16.4 ± 1.0 28.0 ± 0.9 55.6 ± 1.0 0.045 ± 0.003 0.25 ± 0.02 1.45 ± 0.02
OmpR pH 5.6
(E.coli)
23 1644 24.3 ± 0.9 39.4 ± 3.7 36.2 ± 4.2 0.066 ± 0.003 0.55 ± 0.06 2.06 ± 0.14
OmpR pH 7.2
(E.coli)
22 478 15.3 ± 1.2 45 ± 1.8 39.7 ± 2.4 0.057 ± 0.005 0.40 ± 0.03 2.06 ± 0.09