Table 1. Apparent diffusion coefficient D obtained from SptPALM of SsrB mutants.
No. of cells | No. of tracks | F1 (%) | F2 (%) | F3 (%) |
D1
(µm2s−1) |
D2
(µm2s−1) |
D3
(µm2s−1) |
|
---|---|---|---|---|---|---|---|---|
Fixed | 41 | 496 | 26.9 ± 4.0 | 65.0 ± 3.7 | 8.1 ± 0.6 | 0.020 ± 0.002 | 0.066 ± 0.003 | 0.88 ± 0.08 |
WT pH5.6 | 89 | 2618 | 28.1 ± 2.6 | 34.5 ± 2.3 | 37.3 ± 0.5 | 0.041 ± 0.002 | 0.14 ± 0.01 | 1.49 ± 0.02 |
WT pH7.2 | 71 | 569 | 11.3 ± 1.1 | 22.2 ± 1.0 | 66.5 ± 1.2 | 0.037 ± 0.004 | 0.25 ± 0.03 | 1.53 ± 0.03 |
K179A pH5.6 | 113 | 1038 | 11.3 ± 1.3 | 18.7 ± 1.2 | 69.9 ± 1.5 | 0.056 ± 0.006 | 0.32 ± 0.05 | 1.70 ± 0.03 |
D56A pH5.6 | 109 | 1345 | 20.5 ± 1.4 | 24.0 ± 1.1 | 55.5 ± 1.2 | 0.045 ± 0.003 | 0.26 ± 0.03 | 1.61 ± 0.03 |
D56A pH7.2 | 74 | 384 | 13.0 ± 1.5 | 27.3 ± 1.4 | 59.8 ± 1.9 | 0.050 ± 0.005 | 0.31 ± 0.04 | 1.66 ± 0.04 |
D56E pH5.6 | 111 | 796 | 11.1 ± 0.8 | 16.2 ± 1.7 | 72.8 ± 2.2 | 0.053 ± 0.004 | 0.39 ± 0.06 | 1.52 ± 0.03 |
PAmCherry pH5.6 | 53 | 715 | 5.4 ± 0.3 | 27.8 ± 1.3 | 66.7 ± 1.4 | 0.029 ± 0.004 | 0.67 ± 0.04 | 3.61 ± 0.07 |
PhoP pH 5.6 | 81 | 3665 | 9.8 ± 1.5 | 23.7 ± 1.3 | 66.4 ± 0.4 | 0.033 ± 0.004 | 0.14 ± 0.01 | 1.54 ± 0.01 |
PhoP pH 7.2 | 103 | 2930 | 10.6 ± 0.6 | 18.8 ± 1.5 | 70.6 ± 1.8 | 0.043 ± 0.003 | 0.40 ± 0.04 | 1.58 ± 0.03 |
OmpR pH 5.6 | 43 | 1610 | 21.9 ± 1.4 | 20.9 ± 1.0 | 57.0 ± 1.3 | 0.054 ± 0.003 | 0.26 ± 0.03 | 1.34 ± 0.02 |
OmpR pH 7.2 | 55 | 2119 | 16.4 ± 1.0 | 28.0 ± 0.9 | 55.6 ± 1.0 | 0.045 ± 0.003 | 0.25 ± 0.02 | 1.45 ± 0.02 |
OmpR pH 5.6 (E.coli) |
23 | 1644 | 24.3 ± 0.9 | 39.4 ± 3.7 | 36.2 ± 4.2 | 0.066 ± 0.003 | 0.55 ± 0.06 | 2.06 ± 0.14 |
OmpR pH 7.2 (E.coli) |
22 | 478 | 15.3 ± 1.2 | 45 ± 1.8 | 39.7 ± 2.4 | 0.057 ± 0.005 | 0.40 ± 0.03 | 2.06 ± 0.09 |