Table 2. Strains and plasmid used in this study.
| Strain number/plasmid | Description/genotype | Reference/Source |
|---|---|---|
| Salmonella strains | ||
| AL63 | Wild-type Salmonella Typhimurium 14028 s | Lab stock |
| AL142 | ssrA::tetRA (TetRA) | (Winardhi et al., 2015) |
| AL160 | ssrA::tetRA attB:: ssrA-PAmCherry (CmR) | This work |
| AL123 | ssrB::ssrB-4-PAmCherry (TetRA) | This work |
| AL125 | ssrB::ssrB-10-PAmCherry (TetRA) | This work |
| AL159 | attB:: PssrB-PAmCherry (CmR) | This work |
| AL60 | ΔssrB | Lab stock |
| AL89 | ΔssrB attB::PAmCherry-4-ssrB (CmR) | This work |
| AL92 | ΔssrB attB::PAmCherry-10-ssrB (CmR) | This work |
| AL286 | ΔssrB attB::PAmCherry-4-ssrB D56A (CmR) | This work |
| AL489 | ΔssrB attB::PAmCherry-4-ssrB D56E (CmR) | This work |
| AL289 | ΔssrB attB::PAmCherry-4-ssrB K179A (CmR) | This work |
| AL518 | phop::kanR | This work |
| AL522 | phop::kanR attB::PAmCherry-4-ssrB (CmR) | This work |
| AL325 | ΔphoP | This work |
| AL391 | ΔphoP attB::phoP-4-PAmCherry (CmR) | This work |
| AL394 | ΔphoP attB::phoP-10-PAmCherry (CmR) | This work |
| AL520 | ompR::kanR | This work |
| AL524 | ompR::kanR attB::PAmCherry-4-ssrB (CmR) | This work |
| AL525 | ΔompR | This work |
| E. coli strains | ||
| AL53 | BW25141 pir+ | (Bijlsma and Groisman, 2005) |
| AL217 | BL21 (DE3) | Lab stock |
| Plasmids vectors | ||
| pKD46 | λ-Red recombinase expression plasmid (AmpR). | (Kenney, 2018) |
| pCP20 | Plasmid containing FLP recombinase for removal of CmR resistance marker flanked by FRT sites (AmpR). | (Kenney, 2018) |
| pKD3 | Plasmid used as a template for amplifying the CmR resistance cassette for constructing gene knockouts in Salmonella (CmR). | (Kenney, 2018) |
| pINT-ts | CRIM helper plasmid (AmpR). | (Bijlsma and Groisman, 2005) |
| pCAH63 | CRIM cloning vector (CmR). | (Bijlsma and Groisman, 2005) |
| pAL-4-PAmCherry | CRIM cloning vector containing the 4XGGSG linker upstream of PAmCherry (CmR). | This work |
| pAL-10-PAmCherry | CRIM cloning vector containing the 10XGGSG linker upstream of PAmCherry (CmR). | This work |
| pAL-PAmCherry-4-SsrB | CRIM plasmid containing the N-terminal PAmCherry-4XGGSG-ssrB fusion (CmR). | This work |
| pAL-PAmCherry-4-SsrB D56A | CRIM plasmid containing the N-terminal PAmCherry-4XGGSG-ssrB D56A fusion (CmR). | This work |
| pAL-PAmCherry-4-SsrB D56E | CRIM plasmid containing the N-terminal PAmCherry-4XGGSG-ssrB D56E fusion (CmR). | This work |
| pAL-PAmCherry-4-SsrB K179A | CRIM plasmid containing the N-terminal PAmCherry-4XGGSG-ssrB K179A fusion (CmR). | This work |
| pAL-PssrB-PAmCherry | CRIM plasmid with a 500 bps 5’UTR fragment containing the ssrB promoter cloned upstream of PAmCherry. | This work |
| pAL-PAmCherry-10-SsrB | CRIM plasmid containing the N-terminal PAmCherry-10XGGSG-ssrB fusion (CmR). | This work |
| pAL-SsrA-10-PAmCherry | CRIM plasmid containing the C-terminal ssrA-10XGGSG-PAmCherry fusion (CmR). | This work |
| pAL-PhoP-4-PAmCherry | CRIM plasmid containing the C-terminal phoP-4XGGSG-PAmCherry fusion (CmR). | This work |
| pAL-PhoP-10-PAmCherry | CRIM plasmid containing the C-terminal phoP-10XGGSG-PAmCherry fusion (CmR). | This work |
| pMPM-A5Ω | pBR322 ori araC PBAD promoter Ω-interposon MCS (AmpR) | Lab stock |
| pMPM-A5Ω-his-ssrB | pMPM-A5Ω plasmid cloned with 6xhis-ssrB (AmpR) | (Arnoldini et al., 2014) |
| pMPM-A5Ω-his-ssrB-D56A | pMPM-A5Ω plasmid cloned with 6xhis-ssrB D56A (AmpR) | (Arnoldini et al., 2014) |
| pMPM-A5Ω-his-ssrB-D56E | pMPM-A5Ω plasmid cloned with 6xhis-ssrB D56E (AmpR) | This work |
| pMPM-A5Ω-his-ssrB-K179A | pMPM-A5Ω plasmid cloned with 6xhis-ssrB K179A (AmpR) | (Chakraborty et al., 2017) |
| pMPM-A5Ω-his-ssrBc | pMPM-A5Ω plasmid cloned with 6xhis-ssrBc (AmpR) | (Arnoldini et al., 2014) |
| pMPM-A5Ω-his-phoP | pMPM-A5Ω plasmid cloned with 6xhis-phoP (AmpR) | This work |
| pKF61 | Plasmid pKLC-II containing the promoter fragment of sseI fused to lacZ (AmpR) | (Arnoldini et al., 2014) |
| pKF8A | Plasmid pMC1871 containing the promoter fragment of ssrB fused to lacZ (TetRA) | (Gao et al., 2017) |
*Antibiotic resistance markers are expressed as follows: TetRA; tetracycline resistance, AmpR; ampicillin resistance, CmR; chloramphenicol resistance, KanR: Kanamycin resistance.