Figure 3. IP3 sequestration does not impair NMJ growth.
(A–E) NMJs were co-stained with anti-DLG (red) and anti-Synapsin antibodies (green) to visualize synaptic boutons, with anti-HRP (blue) to visualize presynaptic membranes. Genotypes or conditions as indicated. All scale bars, 10 μm. (F) NMJ growth was assessed by bouton counting at abdominal segments A2 and A3, muscle 6/7, based on postsynaptic DLG staining and double checked for presynaptic Synapsin. No statistically significant differences in NMJ growth versus driver control were observed for any of the experimental conditions (p>0.1 vs. control, regardless of segment). (G) Bouton counts were normalized per unit of muscle 6/7 area. No statistically significant differences versus control were observed (p>0.2 vs. control, regardless of segment). Violin plots used as in Figure 1. For both F and G, data were compared for each segment individually using the Kruskal-Wallis ANOVA test followed by Dunn’s multiple comparisons test.