Figure 4. Fimbrin Fim1 and α-actinin Ain1 competition at actin patches is driven by their residence time on F-actin.

(A,B, top) Fluorescence micrographs of fission yeast in an ain1-1Δ background overexpressing GFP-tagged wild-type Ain1 (A) or mutant Ain1(R216E) (B) from the 41Xnmt1 promoter. Scale bars, 5 μm. (A,B, bottom) Timelapse (in s) of cell taken from a single Z-plane. (C) Percentage of cells in which Ain1-GFP is observed in actin patches. Error bar, s.e. Two-tailed t-test for data sets with unequal variance yielded p-value=0.0029.

Figure 4—video 1. Overexpressed mutant α-actinin Ain1(R216E)-GFP, but not Ain1-GFP, localizes to actin patches in the presence of fimbrin Fim1, related to Figure 4.

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DOI: 10.7554/eLife.47279.011

(Top) Fission yeast cells with Ain1-GFP overexpressed under the 41xnmt promoter (left) and expressing Fim1-mCherry at the endogenous locus (right). (Bottom) Fission yeast cells with Ain1(R216E)-GFP overexpressed under the 41xnmt promoter and expressing Fim1-mCherry at the endogenous locus. Scale bar, 5 μm. Time in s.