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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Food Chem Toxicol. 2019 May 9;130:219–230. doi: 10.1016/j.fct.2019.05.012

Figure 6.

Figure 6

Pyocyanin results in sustained and robust reductions in body weight, lean and fat mass of female C57BL/6J mice. Female mice received three IP injections of vehicle (VEH) or pyocyanin (PYO, 40 mg/kg) one week apart with study endpoint occurring 2 weeks after the third injection. (A) Body weights (g). (B) Percent lean mass (lean mass (g) / body weight (g) x 100)). (C) Percent fat mass (fat mass (g) / body weight (g) x 100)). (D) Ex vivo lipolysis of EF explants as quantified by glycerol release. (E) mRNA abundance of CYP1a1, PPARγ, aP2, TNF-α, F4/80, or RANTES in EF, RPF, and liver. (F) Oil Red O (ORO) absorbance (510 nm) of adipocytes differentiated from SVF cells of male mice administered vehicle (VEH) or pyocyanin (PYO). (G) mRNA abundance of target genes in adipocytes differentiated from (F). ↓, indicates timing of IP injection. Data are mean ± SEM from n = 5 mice/group. *, P<0.05 compared to VEH. #, P<0.05 as compared to baseline pyocyanin.