Table 1.

K_M, k_cat and specificity constant (k_cat/K_M) for Nudt8 with different CoA substrates. The kinetic parameters K_M and k_cat were determined, ± SEM, by non-linear regression analysis of data obtained from 3 independent experiments, each conducted in duplicate.

Substrate	KM, µM	kcat, min−1	kcat/KM, min−1*µM−1
Free CoA	150 ± 13	216 ± 8	1.44
Acetyl-CoA	707 ± 252	116 ± 10	0.16
Propionyl-CoA	318 ± 87	155 ± 14	0.49
Butyryl-CoA	224 ± 40	107 ± 9	0.48
Malonyl-CoA	233 ± 71	184 ± 19	0.79
Succinyl-CoA	329 ± 72	187 ± 14	0.57
Hexanoyl-CoA	251 ± 98	149 ± 11	0.59