Splenocytes were harvested on day 25 from EAE mice treated in vivo with PBS, PLP, ETPGS alone, or ETPGS+PLP. Splenocytes were incubated for 96h with or without a 25 μM PLP rechallenge. Supernatant cytokine levels of A.) GM-CSF, B.) IFN-γ, C.) Il-2, D.) Il-21, E.) Il-6, F.) Il-10, G.) Il-15, H.) Il-17, I.) Il-23, and J.) TNF-α were determined and K.) cell metabolism was measured via resazurin. (n = 5–6 mice/group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p< 0.0001). Error bars are reported as standard deviation among samples.