Value of the data •Multidisciplinary data set obtained from three farm locations across Newfoundland containing podzolic soils with varying pH levels •Data set involves biochemistry, dairy science and agriculture which demonstrate the importance of root lipid in producing higher quality forage on podzolic soils in cool climates of boreal ecosystem •Data set depict the importance of membrane lipid remodeling for sustainable crop production on podzolic soils in boreal ecosystem as part of a strategy to improve food security in the Boreal or northern regions •Data set demonstrates similar result trends and add more context to the results observed in the research article by Nadeem et al., [1].