Fig. 4.

CMV and HIV antigen presentation drives MDC1-mediated HIV-1 latency reversal. a) MDC1 were loaded with either CMV pp65, HIV-1 Gag, or influenza M1 antigen and tested for their ability to induce LR in autologous CD4⁺ T cells. Culture supernatants were assayed by qRT-PCR for detection of HIV-1 RNA at day 7. b) Representative flow cytometry plots of p24 expression of day 20 cocultures, gated on total CD4⁺ T cells. c) Graphical representation of MDC1/antigen-induced CD4 downregulation in cocultures described in (b), as measured by flow cytometry. d) Expression of p24 expression in cell populations detailed in (b). Each colour in (c) and (d) represents an individual study participant. P values comparing viral RNA levels were determined by multilevel mixed-effects tobit regression analyses. Error bars indicate mean ± SEM. *P<0.05, **P<0.01, and ***P<0.001.