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. 2019 Mar 1;8(1):1–8. doi: 10.1016/j.cr.2019.01.002

Fig. 3.

Fig. 3

Intracortically grafted NPCs received synaptic inputs from host neurons at 3 mpt and 6 mpt. A. Representative images of brain slices at 3 mpt. GFP+/mCherry+ cells represent grafted cells infected by viruses, while GFP-/mCherry+ cells represent host neurons. The STEM121 antibody, HNA antibody and GFP fluorescence was used to confirm the location of grafted cells. Green dots represent grafted cells. B. Fluorescence photomicrographs showed more GFP-/mCherry+ host cells in the corpus callosum, hippocampus and thalamus at 6 mpt than at 3 mpt. Immunostaining of NeuN indicated that GFP-/mCherry+ host cells were neurons. Green dots represent grafted cells.