Fig. 3.

SM mast cells aberrantly express CD45RA. (a) Enzymatic staining demonstrates tryptase expression, and MGG staining reveals granules in SM mast cells. The images are representative of two experiments (samples SM32 and SM33). Images were captured using an Olympus XC10 camera (Olympus, Tokyo, Japan). The image width corresponds to 29 μm. (b) Mast cell frequencies in Ctrl, ISM, AdvSM, and MDS/MPN patients, showing the median of log-transformed values. (c) Histograms showing CD45RA expression in mast cells (red line), FMO controls (grey histogram) and CD34⁺ cells (dotted line). (d) Frequency of CD45RA-expressing mast cells, showing the mean and SEM. Control subjects included three subjects with CM (open circles). Supplementary Table 1 shows the characteristics of the indicated subjects. One-way ANOVA with Tukey's multiple comparison was used in panels b and d, *P < .05, **P < .01, **** P < .0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)