Fig. 1. Differences in body weight, dorsal ear skin thickness and phenotypes, as well as back skin phenotypes. Body weights of mice in the 5 groups were measured using a chemical balance (A). PA solution was repeatedly applied to the dorsal ear and back skin during the topical application of Centella asiatica phytosome. After 4 weeks, dorsal ear skin thickness (B), the number of times scratching at the end of the application experiment (C) and phenotypes (D) were observed following the procedure described in Materials and Methods.

Data shown are the mean ± standard deviation (n = 10).

Con, control; PA, phthalic anhydride; TECA, titrated extract of Centella asiatica; AST, astaxanthin.

^*P < 0.05 is the significance level compared to the control group. ^†P < 0.05 is the significance level compared to the PA treatment group. ^‡P < 0.05 is the significance level compared to the TECA or AST treatment alone group.