Table 2.

Primers used in this study and their amplification efficiency and amplification characteristics.

Genes	Primer sequence (5′-3′)	Tm (°C)	R2	E (%)	Amplicon size (bp)
ACT1	F: CGACAATGGAACTGGAATGGTTA	59	0.997	0.936	296
	R: GTGCCTCGGTAAGTAGAATAGGA	59
ACT2	F: TGTGCCAATCTACGAGGGTT	59	0.988	0.934	137
	R:TTTCCCGTTCTGCTGTTGTG	59
ALDH	F: ACAACGGCACTACTGGATCA	59	0.994	0.920	224
	R: CCAATGCAAGCCTGTCCATT	59
EF1B	F: GCAACAATGGCCGTTACCT	59	0.996	0.903	153
	R: ATCGCTGGGTTTCACTGGA	59
GAPDH	F:CTCCCATGTTTGTTGTTGGTGTCA	62	0.997	0.951	217
	R: CTTCCACCTCTCCAGTCCTTCATT	62
HAF1	F: TAAGTCCACGTCTAAGATCAGCA	59	0.997	0.938	186
	R: GCCTGGTTTCTCTCTGTACTAGA	59
LOS1	F: GGCAAGAGACTGTGGAGGA	59	0.999	0.909	162
	R: CAGCAACACGAACAACAGGA	59
UBC35	F: GAGGATCACGTCAGATTCGAG	58	0.998	0.907	163
	R: AGTTTCCTTGATGATTCTTCGCG	60
UBQ9	F: GTGCTGAGAGATGCGGATTG	59	0.998	0.908	252
	R: CCTCTCCTCCTCCAACAGTC	59
UEP	F: GAGGTCGAGAGCAGTGACAC	60	0.998	0.991	178
	R: CCCTGAGCCTCAACACAAGA	60
KUP9	F: TGTTCTTTCGGCAACTGGTG	59	1.000	0.979	284
	R: AAATCCAACCATCCCGACCT	59