Table 3.
Expression stability values (M) of 10 candidate reference genes calculated by NormFinder.
| Rank | Drought | Heat | Cold | Salt | Tissues | All samples |
|---|---|---|---|---|---|---|
| 1 | UEP | UBQ9 | GAPDH | GAPDH | UBC35 | GAPDH |
| Stability | 0.214 | 0.196 | 0.269 | 0.162 | 0.299 | 0.479 |
| 2 | HAF1 | GAPDH | EF1B | ACT1 | ACT1 | UBQ9 |
| Stability | 0.271 | 0.451 | 0.291 | 0.380 | 0.375 | 0.601 |
| 3 | LOS1 | ALDH | UBC35 | HAF1 | UBQ9 | UBC35 |
| Stability | 0.323 | 0.463 | 0.295 | 0.439 | 0.442 | 0.670 |
| 4 | UBQ9 | ACT1 | ACT2 | ACT2 | GAPDH | UEP |
| Stability | 0.398 | 0.556 | 0.421 | 0.507 | 0.445 | 0.858 |
| 5 | ACT1 | EF1B | UBQ9 | UEP | ALDH | ACT1 |
| Stability | 0.451 | 0.610 | 0.519 | 0.557 | 0.560 | 0.871 |
| 6 | ALDH | UEP | LOS1 | LOS1 | UEP | EF1B |
| Stability | 0.474 | 0.612 | 0.698 | 0.603 | 0.612 | 1.173 |
| 7 | EF1B | UBC35 | UEP | UBC35 | EF1B | ACT2 |
| Stability | 0.490 | 0.673 | 0.725 | 0.640 | 0.617 | 1.253 |
| 8 | GAPDH | LOS1 | ALDH | EF1B | ACT2 | HAF1 |
| Stability | 0.513 | 0.715 | 0.780 | 0.647 | 0.773 | 1.340 |
| 9 | UBC35 | ACT2 | ACT1 | UBQ9 | HAF1 | ALDH |
| Stability | 0.529 | 0.868 | 0.917 | 0.729 | 0.911 | 1.344 |
| 10 | ACT2 | HAF1 | HAF1 | ALDH | LOS1 | LOS1 |
| Stability | 0.629 | 0.922 | 1.047 | 0.735 | 1.492 | 1.777 |