Table 4.
Expression stability values (M) of 10 candidate reference genes calculated by BestKeeper.
| Rank | Drought | Heat | Cold | Salt | Tissues | All samples |
|---|---|---|---|---|---|---|
| 1 | LOS1 | UBQ9 | GAPDH | GAPDH | UBC35 | GAPDH |
| r value | 0.983 | 0.956 | 0.966 | 0.966 | 0.966 | 0.929 |
| 2 | UEP | ACT1 | EF1B | ACT1 | UEP | ACT1 |
| r value | 0.980 | 0.935 | 0.896 | 0.952 | 0.965 | 0.916 |
| 3 | HAF1 | GAPDH | ALDH | LOS1 | ACT1 | UBQ9 |
| r value | 0.975 | 0.910 | 0.895 | 0.934 | 0.933 | 0.909 |
| 4 | ACT1 | EF1B | LOS1 | UBQ9 | UBQ9 | UEP |
| r value | 0.971 | 0.901 | 0.873 | 0.919 | 0.921 | 0.901 |
| 5 | ALDH | ALDH | UBC35 | EF1B | GAPDH | UBC35 |
| r value | 0.955 | 0.882 | 0.869 | 0.916 | 0.910 | 0.892 |
| 6 | GAPDH | LOS1 | ACT1 | UEP | HAF1 | HAF1 |
| r value | 0.950 | 0.802 | 0.862 | 0.874 | 0.878 | 0.788 |
| 7 | UBQ9 | UEP | ACT2 | HAF1 | ALDH | EF1B |
| r value | 0.947 | 0.792 | 0.837 | 0.866 | 0.853 | 0.782 |
| 8 | EF1B | ACT2 | UBQ9 | ACT2 | EF1B | ALDH |
| r value | 0.925 | 0.775 | 0.746 | 0.846 | 0.833 | 0.751 |
| 9 | UBC35 | UBC35 | UEP | UBC35 | ACT2 | ACT2 |
| r value | 0.908 | 0.752 | 0.584 | 0.730 | 0.746 | 0.748 |
| 10 | ACT2 | HAF1 | HAF1 | ALDH | LOS1 | LOS1 |
| r value | 0.871 | 0.727 | 0.001 | 0.717 | 0.001 | 0.563 |