Table 5.
Ranking of expression stability for the 10 candidate reference genes.
| Methods | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| A. Drought stress (Ranking order from better-good-average) | ||||||||||
| geNorm | GAPDH/ALDH | HAF1 | LOS1 | UEP | ACT1 | UBQ9 | EF1B | UBC35 | ACT2 | |
| NormFinder | UEP | HAF1 | LOS1 | UBQ9 | ACT1 | ALDH | EF1B | GAPDH | UBC35 | ACT2 |
| Bestkeeper | LOS1 | UEP | HAF1 | ACT1 | ALDH | GAPDH | UBQ9 | EF1B | UBC35 | ACT2 |
| ∆CT | UEP | HAF1 | LOS1 | UBQ9 | ALDH | ACT1 | GAPDH | EF1B | UBC35 | ACT2 |
| Comprehensive | UEP | HAF1 | UBQ9 | LOS1 | ALDH | GAPDH | UBC35 | ACT1 | EF1B | ACT2 |
| B. Heat stress (Ranking order from better-good-average) | ||||||||||
| geNorm | ACT1/GAPDH | UBQ9 | ALDH | UEP | UBC35 | EF1B | LOS1 | ACT2 | HAF1 | |
| NormFinder | UBQ9 | GAPDH | ALDH | ACT1 | EF1B | UEP | UBC35 | LOS1 | ACT2 | HAF1 |
| Bestkeeper | UBQ9 | ACT1 | GAPDH | EF1B | ALDH | LOS1 | UEP | ACT2 | UBC35 | HAF1 |
| ∆CT | UBQ9 | GAPDH | ALDH | ACT1 | UEP | EF1B | UBC35 | LOS1 | ACT2 | HAF1 |
| Comprehensive | UBQ9 | GAPDH | ACT1 | UEP | ALDH | UBC35 | EF1B | LOS1 | ACT2 | HAF1 |
| C. Cold stress (Ranking order from better-good-average) | ||||||||||
| geNorm | UBC35/ACT2 | EF1B | GAPDH | UBQ9 | UEP | LOS1 | ALDH | ACT1 | HAF1 | |
| NormFinder | GAPDH | EF1B | UBC35 | ACT2 | UBQ9 | LOS1 | UEP | ALDH | ACT1 | HAF1 |
| Bestkeeper | GAPDH | EF1B | ALDH | LOS1 | UBC35 | ACT1 | ACT2 | UBQ9 | UEP | HAF1 |
| ∆CT | GAPDH | EF1B | UBC35 | ACT2 | UBQ9 | LOS1 | UEP | ALDH | ACT1 | HAF1 |
| Comprehensive | UBC35 | GAPDH | EF1B | ACT2 | UBQ9 | UEP | LOS1 | ALDH | ACT1 | HAF1 |
| D. Salt stress (Ranking order from better-good-average) | ||||||||||
| geNorm | ACT2/UEP | GAPDH | ACT1 | EF1B | LOS1 | HAF1 | UBC35 | ALDH | UBQ9 | |
| NormFinder | GAPDH | ACT1 | HAF1 | ACT2 | UEP | LOS1 | UBC35 | EF1B | UBQ9 | ALDH |
| Bestkeeper | GAPDH | ACT1 | LOS1 | UBQ9 | EF1B | UEP | HAF1 | ACT2 | UBC35 | ALDH |
| ∆CT | GAPDH | ACT1 | HAF1 | ACT2 | UEP | LOS1 | UBC35 | EF1B | ALDH | UBQ9 |
| Comprehensive | GAPDH | ACT1 | ACT2 | UEP | HAF1 | UBC35 | LOS1 | EF1B | ALDH | UBQ9 |
| E. Tissues (Ranking order from better-good-average) | ||||||||||
| geNorm | ACT1/UBQ9 | GAPDH | UEP | ALDH | EF1B | UBC35 | ACT2 | HAF1 | LOS1 | |
| NormFinder | UBC35 | GAPDH | ACT1 | UEP | UBQ9 | EF1B | ACT2 | ALDH | HAF1 | LOS1 |
| Bestkeeper | UBC35 | UEP | ACT1 | UBQ9 | GAPDH | HAF1 | ALDH | EF1B | ACT2 | LOS1 |
| ∆CT | ACT1 | GAPDH | UBQ9 | UEP | UBC35 | EF1B | ALDH | ACT2 | HAF1 | LOS1 |
| Comprehensive | ACT1 | GAPDH | UBC35 | UBQ9 | ACT2 | UEP | EF1B | ALDH | HAF1 | LOS1 |
| F. All samples (Ranking order from better-good-average) | ||||||||||
| geNorm | GAPDH/UBQ9 | ACT1 | UBC35 | UEP | ALDH | EF1B | ACT2 | HAF1 | LOS1 | |
| NormFinder | GAPDH | UBQ9 | UBC35 | UEP | ACT1 | EF1B | ACT2 | HAF1 | ALDH | LOS1 |
| Bestkeeper | GAPDH | UBQ9 | UBC35 | ACT2 | UEP | EF1B | HAF1 | LOS1 | ACT1 | ALDH |
| ∆CT | GAPDH | UBQ9 | UBC35 | UEP | ACT1 | EF1B | ACT2 | ALDH | HAF1 | LOS1 |
| Comprehensive | GAPDH | UBQ9 | UBC35 | UEP | ACT1 | EF1B | ACT2 | ALDH | HAF1 | LOS1 |