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. 2019 Jun 10;9:8370. doi: 10.1038/s41598-019-44827-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of GR activation on PSORS1C3 and OCT4 expression levels. (A) Location of GRE in PSORS1C3 promoter 0 cloned into PGL4.14 vector. As a control we constructed a mutant promoter in which GRE was deleted by SOEing PCR. (B) Dual luciferase assay showed that the GRE in PSORS1C3 promoter was active and the promoter showed a dose dependent response to dexamethasone treatment. (C) The curve fit indicated that the EC50 value for dexamethasone is 100 nM. (D) The activity of PSORS1C3 promoter 0 in A549 cells decreased after treatment with 100 nM dexamethasone. (E) The inhibitory effect of GR activation on PSORS1C3 transcription was confirmed after treating A549 cells with DEX. The expression of PSORS1C3 long variants dropped significantly 1 h post treatment. Different isoforms of OCT4 gene showed a progressive up regulation after treatment followed by gradual down regulation. The expression alterations of PSORS1C3 short transcripts was similar to OCT4.