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. 2019 Jun 10;9:8386. doi: 10.1038/s41598-019-44924-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene set enrichment analysis of correlations between gene expression and later adaptive immune responses using (a) high-level and (b) low-level blood transcription modules. Expression values for each of 20,525 genes at I1 Day +27/I2 Day −1 (see Fig. S1) were correlated with the percentage of CD107a + CD4+ T cells or CD8+ T cells producing granzyme B after re-stimulation with P. falciparum-infected erythrocytes. Re-stimulated cells were PBMCs obtained a day before challenge (CHMI Day −1). Enrichment analysis were performed on correlations with P < 0.05 and ranked by Spearman rho. Only modules with Benjamini-Hochberg adjusted P values < 0.05 are shown. NES = normalized enrichment score.