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. 2019 Jun 10;10:2538. doi: 10.1038/s41467-019-10386-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Cordycepin prevents senescence and SASP, which are NRF2-dependent. a NRF2 and p-NRF2 expression levels in whole cell lysates from cordycepin-treated fibroblasts for the indicated times. This test was repeated three times. Representative images were shown. b NRF2 expression levels in nuclei from cordycepin-treated fibroblasts for the indicated times. c Representative immunofluorescence pictures of NRF2 in control and cordycepin-treated (3 days) fibroblasts. This test was repeated three times. Representative images were shown. d Representative immunofluorescence pictures of NRF2 of the tongues from normal mice and irradiated mice (control, prevention, treatment) 10 days after radiation. e p-NRF2 expression levels in nuclei from cordycepin-treated fibroblasts for the indicated times. f Quantification of mRNA expression for GSTA1, NQO1 and Srx from cordycepin-treated fibroblasts for the indicated times. g GCLC, GCLM, HMOX1 and NQO1 expression levels in whole cell lysates from cordycepin-treated fibroblasts for the indicated times. h Western blot analysis of p16 and p21 levels in irradiated control or cordycepin-treated (3 days) fibroblasts following knockdown of NRF2 7 days after radiation. i Quantification of mRNA expression for senescence secretory phenotype in fibroblasts following knockdown of NRF2 7 days after radiation. j Staining for SA-β-gal 7 days after radiation in fibroblasts following knockdown of NRF2. This test was repeated three times. Representative images were shown. k Representative images of fibroblasts colonies generated in survival assays following knockdown of NRF2. l Analysis of reactive oxygen species (ROS) levels 24 h after radiation in fibroblasts following knockdown of NRF2. Bars represent 50 μm (c), 100 μm (d), 250 μm (j). Data in f, i, and l represent the means ± S.D. (n = 3, *P < 0.05, **P < 0.01; student’s t-test)