Fig. 6.
Logistic regression analysis- logit values from the blinded samples. The level of plasma miRNAs from 37 HIV-1 positive plasma from the early stage of infection (eclipse stage n = 8, RNA+ stage n = 12, Ag + stage n = 6 and Ag + Ab+ stage n = 11) and 12 healthy controls plasma were examined as blinded samples using real-time RT-PCR and normalized with cel-miR-39 as the control. Logit values were derived from regression analysis (A) PeHIV-1 for detection of early HIV-1 infection. The results showed that early HIV-1 were detected from normal control with an 100% accuracy (B) Pa HIV-1 for detection of acute stage of HIV-1 infection. The results showed that acute HIV-1 were detected from normal control with an 100% accuracy (C) PE for eclipse stage. The results showed that eclipse stage was detected from normal control with an 90.00% accuracy (D) PR, for RNA+ stage. The results showed that RNA+ stage was detected from normal control with an 95.83% accuracy (E) PA for Ag + stage. The results showed that Ag + were detected from normal control with a 72.22% accuracy and (F) PP-SERO for Ag + Ab+ or post-seroconverted stage of HIV-1 infection. The results showed that Ag + Ab+ stage was detected from normal control with an 86.96% accuracy.