Table 1.
Primer sequences of target using qPCR.
| Target gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
|---|---|---|
| CD86 | TAGGGATAACCAGGCTCTAC | CGTGGGTGTCTTTTGCTGTA |
| COX-2 | GCAAATCCTTGCTGTTCCAACC | GGAGAAGGCTTCCCAGCTTTTG |
| iNOS | GCAGAATGTGACCATCATGG | ACAACCTTGGTGTTGAAGGC |
| IL-1β | TGATGTTCCCATTAGACAGC | GAGGTGCTGATGTACCAGTT |
| TNF-α | GTAGCCCACGTCGTAGCAAA | CCCTTCTCCAGCTGGGAGAC |
| IFN-γ | GAGGTGAACAACCCACAGA | TATTGGCACACTCTCTACCC |
| IL-6 | TCTTGGGAC-TGATGCTGGTG | CAGAATTGCCATTGCACAACTC |
| TGF-β1 | TGAGTGGCTGTCTTTTGACG | GGTTCATGTCATGGATGGTG |
| TGF-β2 | CTCCACATATGCCAGTGGTG | CTAAAGCAATAGGCGGCATC |
| TGF-β3 | CCCAACCCCAGCTCCAAGCG | AGCCACTCGCGCACAGTGTC |
| IGF-1 | CAGTTCGTGTGTGGACCAAG | GTCTTGGGCATGTCAGTGTG |
| CCL2 | TTCACTGGCAAGATGATCCC | TGCTTGAGGTGGTTGTGGAA |
| CCR2 | ATGCTGTCCACATCTCGTTCTCG | TTATAAACCAGCCGAGACTTCCTGC |
| G-CSF | CCATTGTCCATCTTGGGGATC | CCTGGAAGCTGTTGTTCCATG |
| GM-CSF | TACCACACCCAGCATTCCTCC | GACCCCTCGCCCAGGTACAGT |
| GAPDH | GTTTGTGATGGGTGTGAACC | TCTTCTGAGTGGCAGTGATG |