Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 4;10:1267. doi: 10.3389/fimmu.2019.01267

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Cevey, Penas, Alba Soto, Mirkin and Goren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cardiomyocytes were transfected with SOCS3 siRNA. After 72 h, cells were treated with 15 μM benznidazole (Bzl) for 30 min and then with 10 mg/L LPS. After 4 h, IL-6 and TNF-α mRNA levels were analyzed by RT-qPCR and normalized against 18S rRNA (A). Concentration of cytokines in culture supernatants was measured by ELISA (B). After 48 h, iNOS mRNA levels were analyzed by RT-qPCR and normalized against 18S rRNA and NO levels were quantified by the Griess reaction in culture supernatants (C). Results are expressed as the mean of three independent experiments (n = 3, 5 replicates/treatment) ± SEM. White bar: Unstimulated control cells. Black bar: LPS stimulated cells. Gray bar: LPS-stimulated and Bzl-treated cells. Hatched gray bar: SOCS3 silenced, LPS-stimulated and Bzl-treated cells. **P < 0.001, vs. LPS-stimulated cells; ^##P < 0.001 vs. control cells.^øP < 0.05 vs. Bzl-treated and LPS-stimulated cells.