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. 2019 Jun 4;10:1267. doi: 10.3389/fimmu.2019.01267

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Copyright © 2019 Cevey, Penas, Alba Soto, Mirkin and Goren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cardiomyocytes were treated with 15 μM of benznidazole (Bzl) for 30 min and then with 10 mg/L LPS. After 24 h, IL-10 and IL-10 receptor (IL-10R) mRNA levels were analyzed by RT-qPCR and normalized against 18S rRNA. Concentration of IL-10 in culture supernatants was measured by ELISA. Results are expressed as the mean of three independent experiments (n = 3, 5 replicates/treatment) ± SEM. White bar: Unstimulated control cells. Black bar: LPS-stimulated cells. Gray bar: LPS-stimulated and Bzl-treated cells.