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. 2019 Jun 4;10:1267. doi: 10.3389/fimmu.2019.01267

Figure 8.

Figure 8

Cardiomyocytes from BALB/c and IL-10-knockout (IL-10-KO) mice of BALB/c background were treated with 15 μM of benznidazole (Bzl) for 30 min and then with 10 mg/L LPS. After 48 h NO levels were quantified by the Griess reaction in culture supernatants (A). Cardiomyocytes from IL-10-KO mice were treated with 50 ng/ml of recombinant mouse IL-10 (rIL-10) for 30 min before Bzl treatment and LPS stimulation. After 48 h NO levels were quantified by the Griess reaction in culture supernatants (B). Results are expressed as the mean of three independent experiments (n = 3, 5 replicates/treatment) ± SEM. White bar: Unstimulated control cells. Black bar: LPS-stimulated cells. Gray bar: LPS-stimulated and Bzl treated cells. Light-Gray bar: rIL-10- and Bzl-treated, LPS stimulated cells. **P < 0.001, vs. LPS-stimulated cells; ##P < 0.001 vs. control cells.øP < 0.05 vs. Bzl treated-LPS-stimulated cells.