Figure 3.

Absence of type I IFN receptor on NK cells allows for a significant increase in IFN-γ production from NK cells. Rag2^−/−γc^−/− splenocytes were isolated and infected with HSV-2 MOI 3. Infected Rag2^−/−γc^−/− splenocytes were then co-cultured with isolated WT or Ifnar^−/− NK cells for 48 h. As controls, Rag2^−/−γc^−/− splenocytes, WT, and Ifnar^−/− NK cells were each infected in separate wells for 48 h. After 48 h of incubation, supernatants were collected and assayed for IFN-γ protein production (n = 3 repeated once with similar results). ^***p < 0.001.