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. 2019 Jun 4;10:1279. doi: 10.3389/fimmu.2019.01279

Figure 5.

Figure 5

(A) Monocytes were stimulated with L-MPLA and treated with liposome-packed Tetanus light chain toxin (L-TeTxLC). The next day lysates for western blot analyses were produced and blottet with anti-TeTxLC-specific and anti-actin- antibodies. (B) L-MPLA-activated, TeTxLC-loaded monocytes with or without L-αSOCS1-siRNA were co-cultured with CFSE-labeled CD3+ T cells from the same donor. After 5–6 days T cell proliferation was determined by flow cytometry (FITC signal). Shown are dot blots and (C) Quantifications of (B) and three more experiments. Shown is the mean + std, n = 4. Statistics: Mann– Whitney U test, one-tailed, confidence intervals 95%, *p ≤ 0.05; ns, not significant. Line above the bars depicts the compared groups. Additionally performed test: Kruskal-Wallis. Number of groups: 5; P-value 0.0729; sum value: not significant; the medians do not vary signif. (P < 0.05). (D) CFSE labeled CD4+ or CD8+ T cells were co-cultured with L-MPLA ± L- αSOCS1 siRNA for 6 days and analyzed at a FACSCanto for fading FITC signal. Shown are the overlays of histogram produced with WEASEL flow cytometry software.