Table 3.
Characteristics of the primary antibodies.
| Antibody | Immunogen | Raised in, mono- vs. polyclonal | Manufacturer | Dilution | Incubation time | |
|---|---|---|---|---|---|---|
| cryosections | inCLARITY | |||||
| IB4-FITC | Griffonia simplicifolia |
Vector (FL-1201) |
1:500 | 16 h | 5 days | |
| IB4-Alexa594 | Griffonia simplicifolia |
Vector (DL-1207) |
1:500 | 16 h | 5 days | |
| CGRP | C-terminus of CGRP protein |
Goat. Polyclonal |
Santa Cruz Biotechnology (sc-8857) |
1:200 | 16 h | 5 days + 2 days secondary Ab |
| Pan Nav | 1501–1518 aa residues of vertebrate Nav1.1 |
Rabbit. Polyclonal |
Alomone Labs (ASC-003) |
1:500 | 16 h | 5 days + 2 days secondary Ab |
| CD68 | Single chain glycoprotein of 110 kDa expressed on the lysosomal membrane of myeloid cells |
Mouse. Monoclonal |
Abcam (ab-31630–125) |
1:400 | 16 h | 5 days + 2 days secondary Ab |
| Tetramethyl-rhodamine | Tetramethylrhodamine fluorophore (FR) |
Rabbit. Polyclonal |
Molecular probes (A6397) |
1:500 | 16 h | — |
| DAPI | DNA | — |
Sigma-Aldrich (D9542) |
1:1000 | 5 min | 16 h |