•Valid and enough evidence and documents were obtained from the feasibility of homemade solutions compared to commercial products in order to use in qualitative fit testing of filtering face piece respirators (FFRs). •This protocol could be used as an essential part of the Respiratory Protection Program (RPP) which could promote the culture of conducting the fit testing procedure regularly, then, it provides higher respiratory protection against hazardous contaminants among the workforces. •This protocol is based on the use of homemade solutions for qualitative fit testing of filtering face piece respirators (FFRs) which would be very useful and cost-benefit for fit testing of the respirator wearers during the emergency situations such as an incidence of a pandemic or other infectious disease outbreaks, and surge capacity events due to unavailability and high cost of the commercial fit test kits.