Figure 2.

Eighteen of twenty antigens elicit antibodies that react with P. falciparum schizonts. Groups of 4 mice were immunized with the proteins in adjuvants as shown in Figure 1. Seven days after the final injection, serum was harvested and pooled for each group before purification of IgG using protein G. The IgG was then probed for reactivity to paraformaldehyde-fixed schizonts in an immunofluorescence assay at a total IgG concentration of 20 μg/mL. All images were captured using the same microscope settings on the same day. Scale bar = 5 μm.